N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is an oxidizable compound that serves as a reducing co-substrate for heme peroxidases. TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm.
T 1505 (OTTO) N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride, 99% Cas 637-01-4 - used as a test reagent in microbiology for the classification of cytochrome oxidase positive aerobic microorganisms. It finds application as a detection of peroxidases on polyacrylamide gels.