B 2225 (OTTO) Bromo thymol blue Cas 76-59-5 - used for measuring pH around 7 in pools and fish tanks. It operates in the pH range 6.0 to 7.6 due to the presence of an electron withdrawing and donating groups like bromine atoms and alkyl substitutents respectively. B 2225 (OTTO) Bromo thymol blue Cas 76-59-5 - used as a biological slide stain as well as used in obstetrics for detecting premature rupture of membranes.